Difference between revisions of "Geranyl diphosphate synthase: Detailed information on the kinetic values obtained from the literature"

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! Description
 
! Description
 
! References
 
! References
 +
|-
 +
| Km<sub>DMAPP</sub>
 +
| 81
 +
| µM
 +
| s110
 +
[[file:24_GPPS_Km_Burke04.png  |300px | An excerpt from Burke, et. al. 2004]]
 +
| <ref name="Burke2004">Burke, C. 2004. [http://www.ncbi.nlm.nih.gov/pubmed/14725857 "Heteromeric geranyl diphosphate synthase from mint: construction of a functional fusion protein and inhibition by biphosphonate substrate analogs", Archives of Biochemistry and Biophysics, 422:52-60]</ref>
 +
|-
 +
| Km<sub>IPP</sub>
 +
| 88
 +
| µM
 +
| s110
 +
[[file:24_GPPS_Km_Burke04.png  |300px | An excerpt from Burke, et. al. 2004]]
 +
| <ref name="Burke2004"></ref>
 +
|-
 +
| Km<sub>DMAPP</sub>
 +
| 241
 +
| µM
 +
|1s10
 +
[[file:24_GPPS_Km_Burke04.png  |300px | An excerpt from Burke, et. al. 2004]]
 +
| <ref name="Burke2004"></ref>
 +
|-
 +
| Km<sub>IPP</sub>
 +
| 215
 +
| µM
 +
|1s10
 +
[[file:24_GPPS_Km_Burke04.png  |300px | An excerpt from Burke, et. al. 2004]]
 +
| <ref name="Burke2004"></ref>
 +
|-
 +
| Km<sub>IPP</sub>
 +
| 44
 +
| µM
 +
| AgGPPS1
 +
[[file:23_GPPS_Km_kcat_Burke02.png  |300px | An excerpt from Burke, et. al. 2002]]
 +
| <ref name="Burke2002">Burke, C. 2002. [http://www.ncbi.nlm.nih.gov/pubmed/12176066 "Geranyl diphosphate synthase from ''Abies grandis'': cDNA isolation, functional expression, and characterization", Archives of Biochemistry and Biophysics, 402:130-136]</ref>
 +
|-
 +
| Km<sub>IPP</sub>
 +
| 55
 +
| µM
 +
| AgGPPS2
 +
[[file:23_GPPS_Km_kcat_Burke02.png  |300px | An excerpt from Burke, et. al. 2002]]
 +
| <ref name="Burke2002"></ref>
 +
|-
 +
| Km<sub>IPP</sub>
 +
| 44
 +
| µM
 +
| AgGPPS3
 +
[[file:23_GPPS_Km_kcat_Burke02.png  |300px | An excerpt from Burke, et. al. 2002]]
 +
| <ref name="Burke2002"></ref>
 +
|-
 +
| Km<sub>DMAPP</sub>
 +
| 163
 +
| µM
 +
| AgGPPS1
 +
[[file:23_GPPS_Km_kcat_Burke02.png  |300px | An excerpt from Burke, et. al. 2002]]
 +
| <ref name="Burke2002"></ref>
 +
|-
 +
| Km<sub>DMAPP</sub>
 +
| 90
 +
| µM
 +
| AgGPPS2
 +
[[file:23_GPPS_Km_kcat_Burke02.png  |300px | An excerpt from Burke, et. al. 2002]]
 +
| <ref name="Burke2002"></ref>
 +
|-
 +
| Km<sub>DMAPP</sub>
 +
| 82
 +
| µM
 +
| AgGPPS3
 +
[[file:23_GPPS_Km_kcat_Burke02.png  |300px | An excerpt from Burke, et. al. 2002]]
 +
| <ref name="Burke2002"></ref>
 +
|-
 +
| Kcat<sub>forward</sub>
 +
| 1.6
 +
| s<sup>-1</sup>
 +
| AgGPPS1
 +
[[file:23_GPPS_Km_kcat_Burke02.png  |300px | An excerpt from Burke, et. al. 2002]]
 +
| <ref name="Burke2002"></ref>
 +
|-
 +
| Kcat<sub>forward</sub>
 +
| 1.8
 +
| s<sup>-1</sup>
 +
| AgGPPS2
 +
[[file:23_GPPS_Km_kcat_Burke02.png  |300px | An excerpt from Burke, et. al. 2002]]
 +
| <ref name="Burke2002"></ref>
 +
|-
 +
| Kcat<sub>forward</sub>
 +
| 0.5
 +
| s<sup>-1</sup>
 +
| AgGPPS3
 +
[[file:23_GPPS_Km_kcat_Burke02.png  |400px | An excerpt from Burke, et. al. 2002]]
 +
| <ref name="Burke2002"></ref>
 +
|-
 +
| Km<sub>DMAPP</sub>
 +
| 390
 +
| µM
 +
| [[file:22_GPPS_Km_Vmax_Schmidt10.png  |400px | An excerpt from Schmidt, et. al. 2010]]
 +
| <ref name="Schmidt2010">Schmidt, A. 2010. [http://www.ncbi.nlm.nih.gov/pubmed/19939949 "A bifunctional geranyl and geranylgeranyl diphosphate synthase is involved in terpene oleoresin formation in ''Picea abies''", Plant Physiology, 152:639-655]</ref>
 +
|-
 +
| Km<sub>IPP</sub>
 +
| 170
 +
| µM
 +
| [[file:22_GPPS_Km_Vmax_Schmidt10.png  |400px | An excerpt from Schmidt, et. al. 2010]]
 +
| <ref name="Schmidt2010"> </ref>
 +
|-
 +
| [IPP]
 +
| 250
 +
| µM
 +
| [[file:22_GPPS_Km_Vmax_Schmidt10.png  |400px | An excerpt from Schmidt, et. al. 2010]]
 +
| <ref name="Schmidt2010"> </ref>
 +
|-
 +
| [DMAPP]
 +
| 800
 +
| µM
 +
| [[file:22_GPPS_Km_Vmax_Schmidt10.png  |400px | An excerpt from Schmidt, et. al. 2010]]
 +
| <ref name="Schmidt2010"> </ref>
 +
|-
 +
| Vmax<sub>forward</sub>
 +
| 4.2
 +
| nkat/mg
 +
| [[file:22_GPPS_Km_Vmax_Schmidt10.png  |400px | An excerpt from Schmidt, et. al. 2010]]
 +
| <ref name="Schmidt2010"> </ref>
 +
|-
 +
| Kcat<sub>forward</sub>
 +
| 1.4
 +
| s<sup>-1</sup>
 +
| [[file:22_GPPS_Km_Vmax_Schmidt10.png  |400px | An excerpt from Schmidt, et. al. 2010]]
 +
| <ref name="Schmidt2010"> </ref>
 
|-
 
|-
 
| Km<sub>DMAPP</sub>
 
| Km<sub>DMAPP</sub>
Line 22: Line 149:
 
| 163
 
| 163
 
| µM
 
| µM
 +
| [[file: 21_GPPS_Km_Mann11.png  |400px | An excerpt from Mann, et. al. 2011]]
 +
| <ref name="Mann2011"> </ref>
 +
|-
 +
| Kcat
 +
| 0.7
 +
| min<sup>-1</sup>
 +
| [[file: 21_GPPS_Km_Mann11.png  |400px | An excerpt from Mann, et. al. 2011]]
 +
| <ref name="Mann2011"> </ref>
 +
|-
 +
| Kcat
 +
| 5.1
 +
| min<sup>-1</sup>
 
| [[file: 21_GPPS_Km_Mann11.png  |400px | An excerpt from Mann, et. al. 2011]]
 
| [[file: 21_GPPS_Km_Mann11.png  |400px | An excerpt from Mann, et. al. 2011]]
 
| <ref name="Mann2011"> </ref>
 
| <ref name="Mann2011"> </ref>

Latest revision as of 16:39, 29 March 2016

Back to terpene model MAIN page.

Back to the GPPS model page.


Detailed description of the values used for the GPPS model

Parameter Value Units Description References
KmDMAPP 81 µM s110

An excerpt from Burke, et. al. 2004

[1]
KmIPP 88 µM s110

An excerpt from Burke, et. al. 2004

[1]
KmDMAPP 241 µM 1s10

An excerpt from Burke, et. al. 2004

[1]
KmIPP 215 µM 1s10

An excerpt from Burke, et. al. 2004

[1]
KmIPP 44 µM AgGPPS1

An excerpt from Burke, et. al. 2002

[2]
KmIPP 55 µM AgGPPS2

An excerpt from Burke, et. al. 2002

[2]
KmIPP 44 µM AgGPPS3

An excerpt from Burke, et. al. 2002

[2]
KmDMAPP 163 µM AgGPPS1

An excerpt from Burke, et. al. 2002

[2]
KmDMAPP 90 µM AgGPPS2

An excerpt from Burke, et. al. 2002

[2]
KmDMAPP 82 µM AgGPPS3

An excerpt from Burke, et. al. 2002

[2]
Kcatforward 1.6 s-1 AgGPPS1

An excerpt from Burke, et. al. 2002

[2]
Kcatforward 1.8 s-1 AgGPPS2

An excerpt from Burke, et. al. 2002

[2]
Kcatforward 0.5 s-1 AgGPPS3

An excerpt from Burke, et. al. 2002

[2]
KmDMAPP 390 µM An excerpt from Schmidt, et. al. 2010 [3]
KmIPP 170 µM An excerpt from Schmidt, et. al. 2010 [3]
[IPP] 250 µM An excerpt from Schmidt, et. al. 2010 [3]
[DMAPP] 800 µM An excerpt from Schmidt, et. al. 2010 [3]
Vmaxforward 4.2 nkat/mg An excerpt from Schmidt, et. al. 2010 [3]
Kcatforward 1.4 s-1 An excerpt from Schmidt, et. al. 2010 [3]
KmDMAPP 58 µM An excerpt from Mann, et. al. 2011 [4]
KmIPP 163 µM An excerpt from Mann, et. al. 2011 [4]
Kcat 0.7 min-1 An excerpt from Mann, et. al. 2011 [4]
Kcat 5.1 min-1 An excerpt from Mann, et. al. 2011 [4]
KmDMAPP 11.60 µM with Co2+ as a cofactor, and IPP as a co-substrate

An excerpt from Frick et al, 2013, Table 1, page 4198

[5]
KmIPP 0.84 µM with Co2+ as a cofactor, and DMAPP as co-substrate

An excerpt from Frick et al, 2013, Table 1, page 4198

[5]
KmDMAPP 1103 µM with Mg2+ as a cofactor, and IPP as co-substrate

An excerpt from Frick et al, 2013, Table 1, page 4198

[5]
KmIPP 11.79 µM with Mg2+ as a cofactor, and DMAPP as co-substrate

An excerpt from Frick et al, 2013, Table 1, page 4198

[5]
[IPP] 15 µM with Co2+ as a cofactor

An excerpt from Frick et al, 2013, Table 1, page 4198

[5]
[DMAPP] 50 µM with Co2+ as a cofactor

An excerpt from Frick et al, 2013, Table 1, page 4198

[5]
Vmaxforward 0.44 µmol min-1mg-1 with Co2+ as a cofactor

An excerpt from Frick et al, 2013, Table 1, page 4198

[5]
Vmaxforward 0.67 µmol min-1mg-1 with Co2+ as a cofactor

An excerpt from Frick et al, 2013, Table 1, page 4198

[5]
Vmaxforward 2.39 µmol min-1mg-1 with Mg2+ as a cofactor

An excerpt from Frick et al, 2013, Table 1, page 4198

[5]
Vmaxforward 0.17 µmol min-1mg-1 with Mg2+ as a cofactor

An excerpt from Frick et al, 2013, Table 1, page 4198

[5]
[IPP] 50 µM with Mg2+ as a cofactor

An excerpt from Frick et al, 2013, Table 1, page 4198

[5]
[DMAPP] 50 µM with Mg2+ as a cofactor

An excerpt from Frick et al, 2013, Table 1, page 4198

[5]

Reference

  1. ↑ 1.0 1.1 1.2 1.3 Burke, C. 2004. "Heteromeric geranyl diphosphate synthase from mint: construction of a functional fusion protein and inhibition by biphosphonate substrate analogs", Archives of Biochemistry and Biophysics, 422:52-60
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 Burke, C. 2002. "Geranyl diphosphate synthase from Abies grandis: cDNA isolation, functional expression, and characterization", Archives of Biochemistry and Biophysics, 402:130-136
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 Schmidt, A. 2010. "A bifunctional geranyl and geranylgeranyl diphosphate synthase is involved in terpene oleoresin formation in Picea abies", Plant Physiology, 152:639-655 Cite error: Invalid <ref> tag; name "Schmidt2010" defined multiple times with different content Cite error: Invalid <ref> tag; name "Schmidt2010" defined multiple times with different content Cite error: Invalid <ref> tag; name "Schmidt2010" defined multiple times with different content Cite error: Invalid <ref> tag; name "Schmidt2010" defined multiple times with different content Cite error: Invalid <ref> tag; name "Schmidt2010" defined multiple times with different content
  4. ↑ 4.0 4.1 4.2 4.3 Mann, F.M. 2011. "Rv0989c encodes a novel (E)-geranyl diphosphate synthase facilitating decaprenyl diphosphate biosynthesis in Mycobacterium tuberculosis", FEBS Letters, 585(3):549-554 Cite error: Invalid <ref> tag; name "Mann2011" defined multiple times with different content Cite error: Invalid <ref> tag; name "Mann2011" defined multiple times with different content Cite error: Invalid <ref> tag; name "Mann2011" defined multiple times with different content
  5. ↑ 5.00 5.01 5.02 5.03 5.04 5.05 5.06 5.07 5.08 5.09 5.10 5.11 Frick, S. 2013. "Metal ions control product specificity of isoprenyl diphosphate synthases in the insect terpenoid pathway", PNAS, 110(11):4194-4199
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