Difference between revisions of "Pyruvate kinase"
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The rate equation is represented by the allosteric regualation model of [http://en.wikipedia.org/wiki/MWC_model Monod, Wyman and Changeux] (MWS). FruBP and Serine are activators and ATP is inhibiting. Simple Micahelis-Menten law (Briggs Haldane) is used for ADP and reverse reaction | The rate equation is represented by the allosteric regualation model of [http://en.wikipedia.org/wiki/MWC_model Monod, Wyman and Changeux] (MWS). FruBP and Serine are activators and ATP is inhibiting. Simple Micahelis-Menten law (Briggs Haldane) is used for ADP and reverse reaction | ||
| − | <center><math>V_m \left( \left(\frac{\frac{[ADP]}{K_{ADP}}}{1+\frac{[ADP]}{K_{ADP}}}\right) \left( \frac{\frac{[PEP]}{K_{PEP}}\left( 1+\frac{[PEP]}{K_{PEP}} \right)^3 }{ \frac{L \left( 1 + \frac{[ATP]}{Ki_{ADP}} \right)^4 }{ \left( 1 + \frac{[SER]}{Ka_{SER}} \right)^4 \left( 1 + \frac{F1,6BP}{Ka_{F1,6BP}} \right)^4 } + \left( 1 + \frac{[PEP]}{K_{PEP}} \right)^4} \right) - \left( \frac{\frac{[ATP][PYR]}{K_{ATP}K_{PYR}K_{eq}}}{\frac{[ATP]}{K_{ATP}} + \frac{[PYR]}{K_{PYR}} + \frac{[ATP][PYR]}{K_{ATP}K_{PYR} + 1 | + | <center><math>V_m \left( \left(\frac{\frac{[ADP]}{K_{ADP}}}{1+\frac{[ADP]}{K_{ADP}}}\right) \left( \frac{\frac{[PEP]}{K_{PEP}}\left( 1+\frac{[PEP]}{K_{PEP}} \right)^3 }{ \frac{L \left( 1 + \frac{[ATP]}{Ki_{ADP}} \right)^4 }{ \left( 1 + \frac{[SER]}{Ka_{SER}} \right)^4 \left( 1 + \frac{F1,6BP}{Ka_{F1,6BP}} \right)^4 } + \left( 1 + \frac{[PEP]}{K_{PEP}} \right)^4} \right) - \left( \frac{\frac{[ATP][PYR]}{K_{ATP}K_{PYR}K_{eq}}}{\frac{[ATP]}{K_{ATP}} + \frac{[PYR]}{K_{PYR}} + \frac{[ATP][PYR]}{K_{ATP}K_{PYR} } + 1} \right) \right)</math></center> |
Revision as of 18:12, 27 February 2014
Pyruvate kinase is a transferase enzyme that catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP.
Chemical reaction
Rate equation
The rate equation is represented by the allosteric regualation model of Monod, Wyman and Changeux (MWS). FruBP and Serine are activators and ATP is inhibiting. Simple Micahelis-Menten law (Briggs Haldane) is used for ADP and reverse reaction
![V_m \left( \left(\frac{\frac{[ADP]}{K_{ADP}}}{1+\frac{[ADP]}{K_{ADP}}}\right) \left( \frac{\frac{[PEP]}{K_{PEP}}\left( 1+\frac{[PEP]}{K_{PEP}} \right)^3 }{ \frac{L \left( 1 + \frac{[ATP]}{Ki_{ADP}} \right)^4 }{ \left( 1 + \frac{[SER]}{Ka_{SER}} \right)^4 \left( 1 + \frac{F1,6BP}{Ka_{F1,6BP}} \right)^4 } + \left( 1 + \frac{[PEP]}{K_{PEP}} \right)^4} \right) - \left( \frac{\frac{[ATP][PYR]}{K_{ATP}K_{PYR}K_{eq}}}{\frac{[ATP]}{K_{ATP}} + \frac{[PYR]}{K_{PYR}} + \frac{[ATP][PYR]}{K_{ATP}K_{PYR} } + 1} \right) \right)](/wiki/images/math/8/f/8/8f80463ba7879b64d7b1bf290f1e5bc8.png)
