Difference between revisions of "Binding of R2 to OR operator"

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|0.1 - 5.6 nM <ref name="Kamionka2004"> Kamionka A, Bogdanska-Urbaniak J, Scholz O, Hillen W. ''Two mutations in the tetracycline repressor change the inducer anhydrotetracycline to a corepressor.'' Nucleic Acids Research. 2004;32(2):842-847.</ref> <ref name="Bolla2012"> Bolla JR, Do SV, Long F, et al. ''Structural and functional analysis of the transcriptional regulator Rv3066 of Mycobacterium tuberculosis.'' Nucleic Acids Research. 2012;40(18):9340-9355. </ref> <ref name="Ahn2007"> Ahn SK, Tahlan K, Yu Z, Nodwell J. ''Investigation of Transcription Repression and Small-Molecule Responsiveness by TetR-Like Transcription Factors Using a Heterologous Escherichia coli-Based Assay.'' Journal of Bacteriology. 2007;189(18):6655-6664.</ref> <ref name="Krok2005"> Sylwia Kedracka-Krok, Andrzej Gorecki, Piotr Bonarek, and Zygmunt Wasylewski. ''Kinetic and Thermodynamic Studies of Tet Repressor−Tetracycline Interaction.'' Biochemistry 2005 44 (3), 1037-1046</ref>
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|<math>0.1 - 5.6 nM </math> <ref name="Kamionka2004"> Kamionka A, Bogdanska-Urbaniak J, Scholz O, Hillen W. ''Two mutations in the tetracycline repressor change the inducer anhydrotetracycline to a corepressor.'' Nucleic Acids Research. 2004;32(2):842-847.</ref> <ref name="Bolla2012"> Bolla JR, Do SV, Long F, et al. ''Structural and functional analysis of the transcriptional regulator Rv3066 of Mycobacterium tuberculosis.'' Nucleic Acids Research. 2012;40(18):9340-9355. </ref> <ref name="Ahn2007"> Ahn SK, Tahlan K, Yu Z, Nodwell J. ''Investigation of Transcription Repression and Small-Molecule Responsiveness by TetR-Like Transcription Factors Using a Heterologous Escherichia coli-Based Assay.'' Journal of Bacteriology. 2007;189(18):6655-6664.</ref> <ref name="Krok2005"> Sylwia Kedracka-Krok, Andrzej Gorecki, Piotr Bonarek, and Zygmunt Wasylewski. ''Kinetic and Thermodynamic Studies of Tet Repressor−Tetracycline Interaction.'' Biochemistry 2005 44 (3), 1037-1046</ref>
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| rowspan="2" |TetR-tetO interaction and  
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| TetR-tetO interaction and  
 
TetR-like Rv3066 from M. tuberculosis
 
TetR-like Rv3066 from M. tuberculosis
 
| rowspan="2" |Repressor protein TetR binds to the operator tetO, repressing its own expression and that of the  
 
| rowspan="2" |Repressor protein TetR binds to the operator tetO, repressing its own expression and that of the  
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|0.6-1.2 <math>min^{-1}</math>
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|<math>0.6-1.2 min^{-1}</math> <ref name="Krok2005"> Sylwia Kedracka-Krok, Andrzej Gorecki, Piotr Bonarek, and Zygmunt Wasylewski. ''Kinetic and Thermodynamic Studies of Tet Repressor−Tetracycline Interaction.'' Biochemistry 2005 44 (3), 1037-1046</ref>
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|SPR of a TetR-like protein on a Gram + GC ~ 50-60%
 
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==References==
 
==References==
 
<references/>
 
<references/>

Revision as of 16:13, 23 September 2015

The ScbR homo-dimer (R2) binds to its own gene promoter (OR) and represses its mRNA transcription.

Chemical equation

O_{R} + 2R_{2} \rightleftharpoons O_{R}-R_{2}

Rate equation

r= \frac{k^{-}_{1}}{K_{d1}}\cdot [O_{R}]\cdot [R_{2}]^{2} - k^{-}_{1}\cdot [O_{R}-R_{2}]

Parameters

Parameter Value Origin Remarks
K_{d1} 0.1 - 5.6 nM [1] [2] [3] [4] TetR-tetO interaction and

TetR-like Rv3066 from M. tuberculosis

Repressor protein TetR binds to the operator tetO, repressing its own expression and that of the

efflux determinant tetA. Similar structure and action with ScbR binding to OR

k^{-}_{1} 0.6-1.2 min^{-1} [4] SPR of a TetR-like protein on a Gram + GC ~ 50-60%

References

  1. ↑ Kamionka A, Bogdanska-Urbaniak J, Scholz O, Hillen W. Two mutations in the tetracycline repressor change the inducer anhydrotetracycline to a corepressor. Nucleic Acids Research. 2004;32(2):842-847.
  2. ↑ Bolla JR, Do SV, Long F, et al. Structural and functional analysis of the transcriptional regulator Rv3066 of Mycobacterium tuberculosis. Nucleic Acids Research. 2012;40(18):9340-9355.
  3. ↑ Ahn SK, Tahlan K, Yu Z, Nodwell J. Investigation of Transcription Repression and Small-Molecule Responsiveness by TetR-Like Transcription Factors Using a Heterologous Escherichia coli-Based Assay. Journal of Bacteriology. 2007;189(18):6655-6664.
  4. ↑ 4.0 4.1 Sylwia Kedracka-Krok, Andrzej Gorecki, Piotr Bonarek, and Zygmunt Wasylewski. Kinetic and Thermodynamic Studies of Tet Repressor−Tetracycline Interaction. Biochemistry 2005 44 (3), 1037-1046
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