Difference between revisions of "Pyrophosphate phosphohydrolase"

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[[Category:No Uncertainty]]
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[[Category:Uncertainty Not Complete]]
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'''Pyrophosphate phosphohydrolase''' that catalyze the reaction that converts Pyrophosphate into two molecules of Phosphate.
 
'''Pyrophosphate phosphohydrolase''' that catalyze the reaction that converts Pyrophosphate into two molecules of Phosphate.
  
 
==Chemical equation==
 
==Chemical equation==
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<center><math>PP \rightarrow 2 P</math></center>
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<center><math>PPi \rightarrow 2 Pi</math></center>
  
 
==Rate equation==
 
==Rate equation==
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|-
 
|-
 
|<math>V_{max}</math>
 
|<math>V_{max}</math>
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|<math>131.25</math>
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|<math>0.36</math>
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|<math>min^{-1}</math>
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|<math>mM/min^{-1}</math>
 
|Human Liver
 
|Human Liver
 
|rowspan="1"|
 
|rowspan="1"|
 
|-
 
|-
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|<math>K_{m}^{PP}</math>
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|<math>K_{m}^{PPi}</math>
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|<math> 0.0054 </math>
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|<math>0.96 </math>
 
|mM
 
|mM
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|Rat liver
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|Human cancer
 
|
 
|
 
|}
 
|}
  
 
==Parameters with uncertainty==
 
==Parameters with uncertainty==
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* The value of <math>V_{max}</math> is reported to be <math>9.6 %</math> of <math>V_{max, PGLM}</math>. The Std. Dev. for <math>V_{max, PGLM}</math> was considered to be <math>10.5%</math> of its mean value. Same error percentage is considered for <math>V_{max}</math>.
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* The minimum and maximum value for <math>K_{m}^{PPi}</math> has been reported to be 0.25 and 1.67. Calculating the mean and Std. Dev. using the '''range formula''' gives a value of <math>0.96 \pm 0.35</math> for this parameter.
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* No Std. Dev. for <math>V_{max}</math> have been reported. Therefore the relative percent standard error is calculated for all the <math>V_{max}</math> which is <math>\approx 71%</math>. Taking this value the Std. Dev. for <math>V_{max}</math> is 0.2556.
  
 
{|class="wikitable"
 
{|class="wikitable"
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|-
 
|-
 
|<math>V_{max}</math>
 
|<math>V_{max}</math>
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|<math>131.25</math>
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|<math>0.36 \pm 0.2556</math>
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|<math>min^{-1}</math>
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|<math>mM/min^{-1}</math>
 
|Human Liver
 
|Human Liver
 
|rowspan="1"|
 
|rowspan="1"|
 
|-
 
|-
−
|<math>K_{m}^{PP}</math>
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|<math>K_{m}^{PPi}</math>
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|<math> 0.0054 </math>
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|<math>0.96 \pm 0.35</math>
 
|mM
 
|mM
 
|Rat liver
 
|Rat liver
 
|
 
|
 
|}
 
|}
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==References==
 
==References==
 
<references/>
 
<references/>

Latest revision as of 13:18, 1 July 2014

Pyrophosphate phosphohydrolase that catalyze the reaction that converts Pyrophosphate into two molecules of Phosphate.

Chemical equation

PPi \rightarrow 2 Pi

Rate equation

Simple Michaelis-Menten rate law is used for this reaction [1]

\frac{V_{max[PP]}}{[PP] + K_{m}^{PP}}


Parameter values

  • A mean body weight is defined as 75Kg in Konig et. al [1]. The V_{max} is calculated by multiplying the reported value with body weight.
Parameter Value Units Organism Remarks
V_{max} 0.36 mM/min^{-1} Human Liver
K_{m}^{PPi} 0.96 mM Human cancer

Parameters with uncertainty

  • The minimum and maximum value for K_{m}^{PPi} has been reported to be 0.25 and 1.67. Calculating the mean and Std. Dev. using the range formula gives a value of 0.96 \pm 0.35 for this parameter.
  • No Std. Dev. for V_{max} have been reported. Therefore the relative percent standard error is calculated for all the V_{max} which is \approx 71%. Taking this value the Std. Dev. for V_{max} is 0.2556.
Parameter Value Units Organism Remarks
V_{max} 0.36 \pm 0.2556 mM/min^{-1} Human Liver
K_{m}^{PPi} 0.96 \pm 0.35 mM Rat liver

References

  1. ↑ 1.0 1.1 M. König, S. Bulik, H.G. Holzhütter (2012), Quantifying the contribution of the liver to glucose homeostasis: a detailed kinetic model of human hepatic glucose metabolism, PLoS Comput. Biol., 8 (6), p. e1002577
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