Transformation of 12-HPETE to 12-HETE

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Upon being generated, 12-HPETE can be reduced by an oxidoreductase enzyme, phospholipid hydroperoxide glutathione peroxidase (PHGPx), to form 12-HETE respectively.

Reaction

R20 HPETE12 - HETE12.jpg

Chemical equation

 12-HPETE \rightleftharpoons 12-HETE

Rate equation

R20.PNG

Parameters

Note that the literature values are the same as reaction 12.

Kms

Literature values
Value Units Species Notes Weight Reference
3.00E-01  mM Human Expression Vector: Bioimprited Enzyme - Selenosubtilisin

Enzyme: Glutathione Peroxidase pH: 7 Temperature: 37

512 [1]
11.1 ± 2.90E-01  mM Human Expression Vector: E Coli

Enzyme: Wild Type Glutathione Peroxidase Enzyme (Se-hGSTZ1-1) pH: 7 Temperature: 37

1024 [2]
4.00E-03  mM Rat Expression Vector: Rat Liver Cells

Enzyme: Glutathione Peroxidase pH: 7.4 Temperature: 37

768 [3]
Description of the PHGPx Kms distribution
Mode (mM) Confidence Interval Location parameter (µ) Scale parameter (σ)
3.19E-01 1.02E+03 2.54E+00 1.92E+00
The estimated probability distribution for PHGPx Kms. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.

Kmp

This is a “Dependent parameter”, meaning that the log-normal distribution for this parameter was calculated using multivariate distributions (this is discussed in detail here). As a result, no confidence interval factor or literature values were cited for this parameter.

Description of the PHGPx Kmp distribution
Mode (mM) Location parameter (µ) Scale parameter (σ)
3.15E-01 2.53E+00 1.92E+00
The estimated probability distribution for PHGPx Kmp. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.

kcat

Literature values
Value Units Species Notes Weight Reference
1170 ± 50  min^{-1} Human Expression Vector: Bioimprited Enzyme - Selenosubtilisin

Enzyme: Glutathione Peroxidase pH: 7 Temperature: 37

512 [1]
24500 ± 150  min^{-1} Human Expression Vector: E Coli

Enzyme: Wild Type Glutathione Peroxidase Enzyme (Se-hGSTZ1-1) pH: 7 Temperature: 37

1024 [2]
Description of the PHGPx kcat distribution
Mode (min-1) Confidence Interval Location parameter (µ) Scale parameter (σ)
2.44E+04 4.20E+00 1.11E+01 9.75E-01
The estimated probability distribution for PHGPx kcat. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.

Enzyme concentration

To convert the enzyme concentration from ppm to mM, the following equation was used.

Literature values
Value Units Species Notes Weight Reference
459  ppm Human Expression Vector: Skin

Enzyme: PHGPx pH: 7.5 Temperature: 37 °C

2048 [4]
307  ppm Human Expression Vector: Lung

Enzyme: PHGPx pH: 7.5 Temperature: 37 °C

1024 [4]
282  ppm Human Expression Vector: Esophagus

Enzyme: PHGPx pH: 7.5 Temperature: 37 °C

1024 [4]
204  ppm Human Expression Vector: Gut

Enzyme: PHGPx pH: 7.5 Temperature: 37 °C

1024 [5]
Description of the PHGPx concentration distribution
Mode (ppm) Mode (mM) Confidence Interval Location parameter (µ) Scale parameter (σ)
3.14E+02 1.74E-03 1.38E+00 5.85E+00 3.09E-01
The estimated probability distribution for PHGPx concentration. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.

Keq

Literature values
Gibbs Free Energy Change Units Species Notes Weight Reference
(-26.941177) kcal/mol Not stated Estimated

Enzyme: PHGPx Substrate:a hydroperoxy-fatty-acyl-[lipid] Product: a hydroxy-fatty-acyl-[lipid] pH: 7.3 ionic strength: 0.25

64 [6]
Description of the PHGPx Keq distribution
Mode Confidence Interval Location parameter (µ) Scale parameter (σ)
5.90E+19 1.00E+01 4.63E+01 8.90E-01
The estimated probability distribution for PHGPx Keq. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.

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